FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005723033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919264
Sequences flagged as poor quality0
Sequence length35-301
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA175031.9040232185748598No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT166961.816235597173391No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG128461.3974222856546106No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC115761.2592682841925713No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA106871.162560483169144No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT104341.1350384655550527No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT93711.0194024785045428No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG93341.0153775194068297No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC88740.9653374873812093No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT84970.9243264176558639No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT76680.8341455773314305No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT69670.7578889198315174No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA69400.7549517875169701No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA65410.7115474988686601No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT63760.6935983569464267No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA55730.606245866258224No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT55300.6015682110906116No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG54270.5903635952240053No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA53440.5813346329237303No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA53080.5774184565043339No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT52960.5761130643645351No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT52560.5717617572318725No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG51110.5559882688759703No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA49800.5417377380165002No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT48300.5254203362690152No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA45670.4968104918717583No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC45290.4926767500957287No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG44980.4893044870679152No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC43420.4723343892505309No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA43260.4705938663974658No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC40700.4427455007484248No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA39270.42718957774915584No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG38800.42207679186827723No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC38170.41522348313433355No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA38160.415114700456017No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG36550.39760068924704983No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC35690.38824537891182515No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC35340.3844379851707453No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC34200.3720367598426567No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG33950.3693171928847426No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG32320.35158561631914226No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC31510.3427742193755004No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC30570.3325486476137432No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG30380.3304817767257284No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC29220.3178629860410067No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC27750.3018719323284714No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC27240.2963240157343266No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG25700.2795714832735754No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC24450.2659736484840046No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC21900.2382340655132802No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC20480.2227869251923278No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC20240.2201761409127302No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC19100.20777491558464165No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC18740.20385873916524525No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC18580.2021182163121802No Hit
TGTCCAGCACGCTTCAGGCTGGGGTGGGGTGGGGGGGCTGTTATCCTTTG16970.18460420510321301No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC16280.17709820029936993No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC16090.17503132941135519No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA15490.16850436871236119No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC12930.1406560030633202No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA12800.13924182824520487No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA12720.13837156681867233No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA11530.12542642809900095No Hit
TGTCCAGCACGCTTCAGGCTGGGGTGGGGTGAGGGACAGGGTCTCCGAAG11380.12379468792425244No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9340.10160302154767291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT405700.0256.83521
TGTCCAG426450.0246.038041
AGCAGTG428400.0242.377352
TCCAGCA437900.0239.614473
GTCCAGC438050.0239.47232
AGCACGC438550.0238.148576
ACGCTTC440500.0235.809229
CACGCTT441000.0235.780698
CCAGCAC449800.0233.041034
CAGCACG449350.0232.922825
GTGGTAT425450.0232.299126
TGGTATC432100.0228.236577
AGTGGTA442500.0224.389715
GCACGCT466750.0223.167857
GCAGTGG467200.0222.0233
GTATCAA446250.0220.468449
CAGTGGT454550.0218.846684
GGTATCA455000.0216.720558
AAGCAGG17250.0176.492251
AGCAGGT17150.0161.9752
Produced by FastQC (version 0.11.5)