FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005723084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779079
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA127121.6316702157290852No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT118341.5189730438119882No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT111601.4324606362127588No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT102221.312062063025701No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA101281.2999965343694286No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT95571.2267048656169657No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC92131.182550165002522No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT80461.0327579103017794No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG79811.024414725592655No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT76260.9788481014120519No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA74000.9498394899618653No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC71890.9227562288291687No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT69920.89746996132613No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA69470.8916939103736591No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT67070.8608883052938148No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA65670.8429183689972389No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA64280.8250767893884958No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA61330.7872115664778541No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA59060.7580745983398346No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA58890.7558925346466789No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA57320.7357405346569476No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC53950.6924843308573329No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA53850.691200763979006No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT49640.6371625984014458No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT47540.610207693956582No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC43020.5521904710562087No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC40360.5180475920927146No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC38430.49327475134100646No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC36300.4659347768326447No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC36190.4645228532664852No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC35920.46105722269500266No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC35870.4604154392558393No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC34360.44103357939310395No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC33660.432048611244816No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG33390.4285829806733335No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC32500.4171592354562246No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC31320.4020131462919678No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30560.39225803801668385No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC29070.3731328915296138No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC27710.35567638198436874No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG26820.3442526367672598No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG26380.3386049425026217No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG26070.33462588517980846No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC26010.33385574505281235No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG24410.3133186749995828No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG23290.2989427259623222No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC23070.2961188788300031No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC21500.27596687884027166No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG20240.25979393617335345No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC19990.2565850189775363No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG19220.24670155401441957No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG18760.2407971463741161No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA17670.22680626740035348No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC17230.2211585731357154No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA13290.17058603812963768No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG12390.15903393622469608No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA11400.1463266241292603No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC11210.1438878470604393No Hit
TGTCCAGCACGCTTCAGGCTGGGGTGGGGTGGGGGGGCTGTTATCCTTCA11140.14298935024561052No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA10490.13464616553648603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT329050.0210.601821
TGTCCAG357600.0204.810231
AGCAGTG344650.0200.62822
GTCCAGC380900.0192.196292
TCCAGCA381250.0191.763523
CAGCACG381800.0190.890035
AGCACGC383000.0190.32036
CCAGCAC386000.0189.347464
GCAGTGG364850.0189.252523
GCACGCT386600.0188.576147
ACGCTTC387800.0187.88069
GTGGTAT354900.0187.857966
CACGCTT388900.0187.460858
TGGTATC358500.0185.456627
AGTGGTA362250.0184.465995
AAGCAGG13100.0181.52651
CAGTGGT373250.0179.43694
GTATCAA371950.0178.779549
GGTATCA374250.0177.709858
AGCAGGT14650.0153.426332
Produced by FastQC (version 0.11.5)