FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005723095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834619
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT170472.042488848204989No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT101261.213248200675997No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT95641.145912086832435No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT94151.128059629603448No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA91741.09918417864918No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC86201.0328065859991207No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT85551.0250186013019116No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA83781.0038113198956649No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA83410.9993781593757152No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA81830.9804473658040375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77460.9280881456089546No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT75720.9072403096502715No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA70860.8490101471449848No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT70110.8400240109558973No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC69780.8360701110326987No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA67690.8110287448524416No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT67180.8049181722438622No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT66230.793535733071018No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT58090.696006201632122No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA57910.6938495289467409No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA57710.6914532259629843No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA56150.6727620626896824No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA52820.6328636180101339No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA51640.6187254304059696No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT44500.5331774138858569No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC41820.5010669539035176No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG39360.47159242720331074No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC38870.46572148489310694No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC38710.4638044425061016No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG37960.4548183063170141No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC37810.45302107907919664No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC36210.4338506552091433No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA35960.43085527647944755No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC35960.43085527647944755No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC35740.4282193431973152No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC33280.3987448164971083No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC32920.3944314711263463No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC30550.36603528076882985No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG29740.3563302536846154No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC29620.35489247189436135No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG29160.34938097503172105No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC29110.3487818992857819No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC28400.34027502369344576No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG28190.33775890556050125No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC27680.33164833295192175No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG26100.31271753938024416No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC25240.3024134365500905No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG24210.2900724761837437No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC23650.28336282782922506No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC23280.27892966730927526No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC22410.26850574932993376No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC21300.2552062677700843No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG21280.2549666374717086No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG21000.25161181329444937No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC20700.24801735881881432No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC20020.23986992867404167No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT16020.19194386899890847No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC15770.18894849026921268No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG14700.17612826930611453No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG10960.13131740350986498No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA10830.12975980657042316No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA10640.1274833187358543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT375950.0224.820921
TGTCCAG334500.0219.118681
AGCAGTG391850.0215.816512
TACGCTT9850.0206.682728
TCCAGCA347150.0205.020583
CAGCACG349200.0203.585135
AGCACGC349700.0203.128656
GTGGTAT397950.0202.596536
CCAGCAC351800.0202.27784
GCAGTGG418500.0201.990433
GTACGCT10100.0201.56687
GTCCAGC353400.0201.394712
GCACGCT354000.0200.49797
ACGCTTC365000.0200.28669
TGGTATC402300.0200.118357
CACGCTT356350.0199.56528
CAGTACG10350.0195.580435
AGTGGTA412850.0195.340745
GTATCAA414700.0193.63259
GGTATCA420200.0191.566048
Produced by FastQC (version 0.11.5)