FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005723096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481981
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT98372.04095182175231No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT73251.5197694514929012No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA63871.32515597087852No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA62431.295279274494223No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT55441.1502528107954464No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT50441.0465142816833028No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT47610.9877982742058298No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC46330.9612412107531211No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC45870.9516972660748038No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC45440.9427757525711594No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT43730.9072971756148064No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43150.8952635062377978No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA37280.7734744730601414No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA35990.7467099325492084No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA35790.7425603913847226No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA35760.7419379602100498No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT35510.7367510337544426No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT34430.7143435114662197No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA34200.7095715391270611No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA31560.6547975957558493No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT31530.6541751645811764No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT31130.6458760822522049No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG30180.6261657617208977No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA29860.6195264958577206No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA27120.562677781904266No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC27050.5612254424966959No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG26600.551888974876603No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC22840.4738776009842712No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG22690.4707654451109069No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG21880.45395980339473957No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA21350.44296351930885236No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC21230.440473794610161No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC20250.4201410429041809No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC20220.419518611729508No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC19690.4085223276436208No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC19560.40582512588670505No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC19100.39628118120838784No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC18910.3923391171021264No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC18660.38715219064651923No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC18410.3819652641909121No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG17960.37262879657081915No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC17440.36183998954315627No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG15530.3222118714223175No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC15180.31495017438446743No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC14950.3101782020453088No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC14740.3058211838225988No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG14680.3045763214732531No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC14370.2981445326683002No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC12430.25789398337278857No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC11530.23922104813260273No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC11250.23341169050232272No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9190.1906714165081196No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG8490.17614802243241953No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAAACCACTGCTT7390.15332554602774798No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG7220.1497984360379351No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG7130.14793114251391654No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA6930.1437816013494308No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA6210.12884325315728212No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAAACCACTGCTT6010.12469371199279639No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA5780.1199217396536378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT206750.0222.238191
AGCAGTG214100.0213.414412
TGTCCAG220250.0213.3661
TCCAGCA231000.0203.185043
CAGCACG230950.0202.625085
AGCACGC231950.0201.601176
CCAGCAC232800.0201.314444
GTCCAGC234200.0200.508072
GCAGTGG228650.0199.93563
GTGGTAT222400.0199.543936
GCACGCT234850.0199.012767
ACGCTTC235350.0198.244239
CACGCTT235600.0198.231228
TGGTATC225950.0196.408817
AGTGGTA231300.0192.167365
GTATCAA230400.0192.110829
CAGTGGT233700.0190.392844
GGTATCA234750.0189.046088
AAGCAGG7500.0187.525991
GCCCGCT4450.0180.229756
Produced by FastQC (version 0.11.5)