FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005723101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738416
Sequences flagged as poor quality0
Sequence length35-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT333001.9155369025595714Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT211851.218638116538274Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT190151.0938118379030106Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT186191.0710324801428428Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT182671.0507841621338045Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT175381.008849435348041Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT175271.0082166754102586Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA174701.0049378284599313Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT163840.9424671655115923Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT158370.9110017395145926Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA157350.9051343291824282Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA151500.8714830052185438Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA142310.8186187885983562Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA142100.8174107923534988Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA138700.7978527579129505Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA135330.7784672943645249Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA133760.7694360843434482Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA128380.7384883710228162Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT124830.7180674821216555Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT124070.713695686187886Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG123810.7122000717894911Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC111890.6436319039861575Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA109990.6327024141517336Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA102620.59030749832031Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC100660.579032866701641Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA98020.5638466281948624Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC91140.5242703702681062Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC89470.5146639239399545Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG88800.5108098406825524Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC82330.47359205161480333Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC81270.46749454675980895Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG75790.4359715971321019Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC74340.42763067067951516Clontech Universal Primer Mix Long (95% over 22bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74310.4274580997873927No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC73790.4244668709906029Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC72160.4150905191852813Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC71530.41146653045070913Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC71010.40847530165391943Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC69990.402607891321755Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG68510.3940943939770457Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC67450.3879968891220513Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC60940.35054900553147234Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC59370.3415177955103957Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG58290.33530524339398626Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC56850.32702184057210704Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG55970.3219597610698475Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG53850.30976475135985865Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC52290.3007910649694895Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG52280.30073354133878194Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC49330.28376407028007106Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG46050.26489631940801284Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC43810.252011026129534Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC43420.24976760453194174Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC39810.22900157384653616Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC37920.2181296076428197Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC37210.21404542986258754Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG36670.21093915380438283Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG34440.19811138415661156Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC26380.15174733780637084Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA24170.13903461542001455Clontech SMART CDS Primer II A (96% over 26bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG23880.1373664301294972No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA19690.11326402886305693Clontech Universal Primer Mix Long (100% over 23bp)
TGTCCAGCACGCTTCAGGCTGGGGTGGGGTGGGGGGGCTGTTATCCTTCA18620.107109000377355No Hit
AAGCAGTGGTTTCACCAGCGTAGGACCAGACTCTCCATCTCTGCGTTGATACCACTGCTT17630.10141416093731304Clontech Universal Primer Mix Long (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)