FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005723102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387511
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT241951.7437699593012235Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT213901.5416093998534064Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC212621.5323842477645222Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA208661.5038439334895364Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT172271.2415757424625824Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG150811.0869103019723807Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC137080.9879561315189572Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA123170.8877046740530345Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC118110.8512364947016636Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT117200.8446779881384724Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC117020.8433807011259731Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC112710.8123178843266828Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG112530.8110205973141835Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT108260.7802460665176708Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT106710.7690749839100375Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG105240.7584804733079593Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA104070.7500481077267135Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC92400.6659406664163383Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA91620.6603190893621744Clontech Universal Primer Mix Long (95% over 22bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGACGACCACGTTCCCA87270.6289679865601066No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA87160.6281752000524681Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA83280.6002114577830374Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG83020.5983375987649827Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC82400.5938691657219294Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG79750.5747702180379111Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT79410.5723197870143012Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG77730.5602117748976404Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA77710.5600676318962516Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT76500.5513469803122282Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG75900.5470226902705636Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA75390.5433470437351487Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC73440.529293101099739Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA69860.5034915038511407Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA69720.5024825028414189Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG69690.5022662883393356Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC60180.43372629117895284Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG59980.43228486116506465Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA59870.4314920746574262Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT56900.4100868389511867Clontech Universal Primer Mix Long (100% over 23bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGGGCTGTTATCCTTTG56060.40403283289285635No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC55230.3980508983352204Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC54340.391636534773418Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC53870.3882491742407808Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC51090.3682132970477351Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC49490.3566818569366297Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC47620.34320448630677525Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC46250.33333069071164123Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC43030.31012366748804154Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA39220.2826644257234718Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC39150.2821599252186109Clontech Universal Primer Mix Long (95% over 22bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG37330.26904291209222847No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC35750.2576556149825119Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC35090.25289889593668086Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT34970.25203403792834794Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC30050.21657485958669875Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC29770.21455685756725532Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA29240.21073706803045167Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG26560.19142190584435007Clontech Universal Primer Mix Long (95% over 22bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26040.1876741878082408No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA21860.15754830051797789Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA20470.14753036192145505Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA17410.12547648270896591Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA16110.11610718761869275Clontech Universal Primer Mix Long (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)