FastQCFastQC Report
Wed 8 May 2024
EGAF00005723116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080633
Total Bases285.6 Mbp
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT364123.3695065762381864Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC293652.7173887897186186Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG239382.2151831380311355Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA198921.8407729543702627Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC126991.1751445680448402Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT124521.1522875944006892Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT118001.091952587048517Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT92030.8516304795430085Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA84460.7815789449332012Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA80090.7411396838704721Clontech Universal Primer Mix Long (100% over 23bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGACGACCACGTTCCCA79190.7328112319353564No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT70020.6479535605520098Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT69340.6416609524232556Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT66420.6146397528115466Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA64280.5948365448769379Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG62590.5791975629098871Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC61670.570684034265102Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG61560.5696661123619212Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC60220.5572659728140821Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG53940.4991518859779407Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA53170.4920264326556749Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC52770.4883248984622901Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC52150.4825875204625438Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT49370.45686185781851935Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG48780.4514020948832767Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG48700.4506617880445997Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC48610.4498289428510882Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT48190.44594233194803423Clontech Universal Primer Mix Long (95% over 22bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGGGCTGTTATCCTTTG47840.44270348952882244No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA46400.4293779664326372Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG46300.428452582884291Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT45630.42225251311037143Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC45570.4216972829813637Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC44290.40985237356253235Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA41390.3830162506604925Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA41250.3817207136928078Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA41130.3806102534347924Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG40500.3747803370802113Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA39850.368765344015961Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA39800.36830265224178793Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA39050.3613622756291914Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC38010.3517382867263909Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG37740.34923975114585615Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC37060.34294714301710205Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA35920.3323977705659553Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG35070.3245320104050126Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC33940.3140751763087006Clontech Universal Primer Mix Long (95% over 22bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33690.31176171743783504No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC33260.30778256817994637Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC30890.2858509780841414Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC30750.28455544111645675Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC29970.2773374494393564Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC29240.2705821495364291Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC28610.26475223318184804Clontech SMART CDS Primer II A (96% over 26bp)
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG26370.24402364169889312No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT23020.21302329282929544Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC22730.21033968053909147Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC22030.20386199570066801Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC20450.18924093563679806Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA19670.1820229439596977Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC18640.17249149341173184Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG17820.164903348315293Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC14920.13806722541325314Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA14760.13658661173589923Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA13190.1220580900268639Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC12620.11678340380129053Clontech Universal Primer Mix Long (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)