FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005723120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005723120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063943
Sequences flagged as poor quality0
Sequence length35-301
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG272022.556715914292401No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT210881.9820610690610303No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG160201.5057197613030022No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG159331.4975426315131544No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC143841.3519521252548303No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT125131.1760968397743112No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT117221.1017507516850056No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA102430.9627395452575938No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC98800.9286211761344358No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA97010.9117969665668181No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT92690.8711932876103325No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT90450.8501395281514142No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT83070.7807749099340848No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT82280.7733497001249128No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG79110.7435548708906398No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA78390.7367875910645588No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA75160.7064288218447793No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA73510.6909204722433439No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG72740.6836832424293406No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA71420.6712765627481923No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG69050.6490009333206761No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC65510.6157284741757781No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC61840.5812341450622825No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGACGACCACGTTCCCA61110.574372875238617No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT59910.5630940755284822No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT58490.5497474958714893No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA55700.5235242865454258No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC55050.5174149367024361No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52390.4924135973449706No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA52110.48978187741260576No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA50590.47549539777976824No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC49660.4667543280044138No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA47080.4425049086276238No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC46960.4413770286566103No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG46800.43987318869525904No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC45930.4316960589054113No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC44120.4146838693426245No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC42320.3977656697774222No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCGGGCTGTTATCCTTTG41190.3871448000503786No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA38290.3598877007508861No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT37460.35208653095137615No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA37340.35095865098036266No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG35960.3379880313137076No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC34490.3241715016687924No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC33190.311952801982813No Hit
TGTCCAGCACGCTTCAGGCTCCCCTCCCCTCCCCAGTAGTCCTTGACCAG30830.28977116255288116No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC29200.2744507929466146No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC27430.25781456337416575No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC26570.2497314235819024No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG24880.2338471139901292No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC23690.22266230427757877No Hit
TGTCCAGCACGCTTCAGGCACAGGTGAGTGAGAGGGTCTGCGACATCGAGCTCTGCGTTGATACCACTGCTT20390.19164560507470796No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC20090.18882590514717423No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC19660.1847843352510426No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACCTGTGCCTGAAGCGTGCTGGACA19640.18459635525587367No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG19600.18422039526553585No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC19470.18299852529693791No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC19360.18196463532350887No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC18210.1711557856012963No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA14110.1326198865916689No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC13700.1287662966907062No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCACCTGAGCCTGAAGCGTGCTGGACA12290.11551370703129774No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA11630.10931036719072355No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA11030.10367096733565613No Hit
TGTCCAGCACGCTTCAGGCTCAGGTGAGGGACAGGGTCTCCGAAGCATCGAGCTCTGCGTTGATACCACTGCTT10980.10320101734773385No Hit
AAGGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCT10930.10273106735981156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCC25950.0390.9172295
GCACGCG25150.0329.46204295
AAGCAGT441900.0246.77911
AGCAGTG464250.0234.106892
GTCCAGC536250.0231.226062
AGCACGC536900.0230.686426
TCCAGCA538800.0230.22583
TGTCCAG539200.0229.913971
CACGCTT539500.0229.715648
ACGCTTC539300.0229.706839
CCAGCAC543950.0227.952874
GTGGTAT470250.0223.357486
CAGCACG563550.0219.709925
TGGTATC479450.0218.807227
AGTGGTA487950.0215.359275
TCGCCAC5650.0212.82292295
GTATCAA494600.0211.874339
CAGTGGT500400.0210.406374
GGTATCA503950.0208.144538
AAGCAGG14950.0206.014141
Produced by FastQC (version 0.11.5)