FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9384921
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG50000.041.4269831
TATGCCG5100.029.30129448
GATCGGA5950.028.621091
CGTATGC6650.024.03766446
ATCGGAA7900.021.9930322
CGTCTGA8900.020.6893616
ACACGTC9300.020.54719413
TCTCGTA7000.020.35211443
CTCGTAT7050.019.7151144
ATGCCGT7450.019.12615649
TGCCGTC7550.018.87353550
GCCGTCT7400.018.78744751
TCGGAAG9550.018.5554713
TCGTATG9300.016.06665245
ACGTCTG11500.016.01176515
ATCTCGT9300.015.31871342
CGTCTTC10150.014.38295753
CGGAAGA12100.014.3577114
CACGTCT13300.013.8447614
CCGTCTT11200.013.34433352
Produced by FastQC (version 0.11.5)