FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712750
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4150.038.52487648
CGTATGC4450.035.92548846
CTCGTAT4600.033.99580444
TCTCGTA5200.030.07189443
CGCTCAT5450.029.32338134
TATCTCG5700.028.04242541
TCGTATG5800.027.56231545
ATGCCGT5800.026.96691149
GATCGGA5550.026.9208681
ATCTCGT6500.024.59169642
ACCGCTC6600.024.21400632
ACACGTC6550.023.86634613
GCCGTCT6700.023.34694551
TGCCGTC6850.023.34172850
GTCACCG6850.022.82210429
ATCGGAA7050.022.6698192
CGTCTGA7550.021.62566216
TCGGAAG8000.020.8456483
CACCGCT7700.020.75467931
CGTCTTC7850.020.37182653
Produced by FastQC (version 0.11.5)