FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421373
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.027.42284248
CGGCTAT4600.022.6431234
GATCGGA5100.021.7903331
CTCGTAT4750.021.20142444
CGTATGC5200.020.03639246
TCTCGTA5100.019.74509443
ATCGGAA6500.017.0950722
ACACGTC7150.016.9946213
GCCGTCT6350.016.96199651
TGCCGTC6150.016.94773750
ACCGGCT6200.016.79928232
TCACCGG6750.016.45870430
TCGTATG7300.015.22338445
TCGGAAG7300.015.2213323
CGTCTGA8250.014.30785116
GTCACCG7650.014.06856129
CACCGGC8150.014.05780431
ATGCCGT7500.013.89573949
TATGCGC2102.8432213E-413.2258339
CCGTCTT9100.012.21924852
Produced by FastQC (version 0.11.5)