FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7230130
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124310.17193328474038502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG61600.048.559451
TATGCCG6550.044.56352248
GATCGGA7250.041.2108651
CGTATGC7700.038.80700746
CTCGTAT7700.037.90005544
TCGTATG8300.035.99918745
GCCGTCT8250.035.38857751
TCTCGTA8150.035.3796743
ATCGGAA8550.035.344372
ATGCCGT8700.034.3521449
GTCACGA8700.033.92920329
ACACGTC9050.033.7686813
TGCCGTC8650.033.74883750
ATCTCGT9300.031.3778842
TCGGAAG9850.031.029053
AGTCACG9950.030.3647828
ACGAGAT9950.030.01742232
CGTCTGA10600.028.50278916
CGTCTTC11100.025.68105353
CACGAGA12150.025.4395131
Produced by FastQC (version 0.11.5)