FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8383012
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.032.92270348
CGTATGC5350.027.93080546
AGGGGGG27200.027.3455221
TTACTCG5900.025.9120735
GATCGGA5550.025.0500371
TCGTATG6600.023.6936645
CTCGTAT6650.022.99253744
TACTCGA6400.022.80202136
TCTCGTA6800.021.97392543
TGCCGTC7150.020.90052250
ATGCCGT7250.020.61211449
CGTCTGA7750.018.82893216
GCCGTCT7850.018.5945151
ATCGGAA8050.018.130912
ATCTCGT8300.017.16508942
ACACGTC8950.016.69317813
TCGGAAG9350.015.6086393
CGTCTTC9850.015.17316553
ACTCGAT9800.014.89120537
CCGTCTT10350.014.77553852
Produced by FastQC (version 0.11.5)