FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7473435
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG36400.015.8314252
GAGGGGG47700.011.7855521
GCGCCTA3000.00559419539.25700446
TGGGGGG76050.07.48614032
AGACGCA5100.00727906646.81776462
GTGGGGG81250.06.5773461
GGGGGGA73800.05.18194821
Produced by FastQC (version 0.11.5)