FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8264955
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA4250.031.06310342
GGTTCCG4800.028.95047841
GCGGTTC6100.024.48688539
CGAAGCT5700.023.16585246
TCCGAAG5950.022.19021244
GCTCGTG6500.021.90519516
TATGCCG3850.021.66551248
TTCCGAA6100.021.64350343
TGCTCGT6500.021.37092215
AAGCGGT6850.020.2831537
TCGTATC4250.019.61559538
CCGAAGC7600.018.74525645
TCTCGTA4650.018.6793143
CTCGTAT4700.018.48149144
AGCGGTT8200.018.21519338
CTGCTCG8550.017.46541814
ATTCGTA5600.016.12633136
GATCGGA5650.015.9853611
CGTATGC5550.015.02649946
TGCCGTC5800.014.38394450
Produced by FastQC (version 0.11.5)