FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8264955
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTC12500.013.6041217
TCGTTCA14950.010.6789218
GTCTGAC15750.010.36633837
GTCATAG18200.09.34898219
CATAGTC18100.08.82508521
CGGCGAA3550.0022119438.81168956
AGTAGTC15850.08.76239815
TAGCAGC19000.08.71043169
ACTAGCA16900.08.10003767
GCCCCAT18450.07.8971741
GGAGCTC22150.07.85001558
GTAGTCA20000.07.812509516
GACTAGC17200.07.71391466
AACGCAC15454.4055923E-97.677226570
CTAGCAG23000.07.637603368
TCATAGT22300.07.63006420
AACGGTA4150.009100237.530677319
AGCAGCT38300.07.52116270
AGTCATA23550.07.0774518
AGGGAGC22150.07.061286456
Produced by FastQC (version 0.11.5)