FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10622963
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG42650.036.31491
TATGCCG5950.026.28366348
CGTATGC6500.024.5900346
CTCGTAT6050.024.11866444
GATCGGA6250.023.8922751
TGCCGTC7150.020.90504350
GCCGTCT8250.020.22660651
ATCGGAA9000.018.1328072
TCGGAAG8900.017.9453953
TCTCGTA8250.017.68585443
TATCTCG8400.017.36749841
CGTCTGA9800.017.00464616
ATGCCGT9200.017.00011649
TCGTATG9150.016.70749745
ACACGTC9900.016.48266413
ATCTCGT9550.014.9131142
CGGAAGA12000.013.8880434
CGTCTTC12850.012.71907153
ACGTCTG13650.012.20846415
CCGTCTT13050.011.98950452
Produced by FastQC (version 0.11.5)