FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4877139
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA2802.512167E-411.1678535
CGCGAAA2500.001223730511.11832936
CGTATGC2550.001441808310.9021146
TTAGCGC2250.007085531510.80848823
TATGCCG2954.06152E-410.602818548
CAACGCG3000.0054767259.280786563
TCCGCGA3100.007270848.96620934
CTCGTAT3700.00322456548.45247244
ACACGTC3700.00323084978.45065213
GCCGTCT4653.2802994E-48.22315851
TCGTATG4653.291559E-48.22054445
TGTGTCG4000.0065168417.81701338
ATGCCGT5200.00108740337.352193449
ACTCCGC4950.00549272177.018714432
ATCTCGT5250.00968578556.618798742
Produced by FastQC (version 0.11.5)