FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7482751
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.020.8513848
CTCGTAT3950.018.4692344
TCTCGTA3850.018.04589743
TAATGCG4250.017.15886534
AATGCGC4700.016.25528735
ATGCGCA4900.015.59211736
CGTATGC5200.014.70043646
TCGTATG5150.014.16733345
ATGCCGT5700.014.02435849
CGCATCT5300.013.76176739
GCGCATC5100.013.61933138
GATCGGA5450.013.3824661
GCCGTCT6000.013.32679751
TGCCGTC6400.013.035650
CGTCTTC5900.012.966953
TGCGCAT5359.094947E-1212.3335237
ACACGTC6500.011.75201513
CGGTTGG3300.00483015911.37948970
ATCGGAA6755.456968E-1210.8045242
ATCTCGT7503.6379788E-1210.18957542
Produced by FastQC (version 0.11.5)