FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9845421
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283420.28786986356398575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG109200.052.559671
TATGCCG7050.040.91850748
GATCGGA7950.036.272391
CTCGTAT8700.033.15371744
TCTCGTA8600.033.13295443
CGTATGC8950.032.6175846
CGCTCAT9100.032.06786334
GTCACCG9450.030.87608129
ATGCCGT9400.030.69044149
ACCGCTC9900.029.12368632
TGCCGTC9900.028.79006250
TCGTATG10050.028.70067245
CACCGCT10600.028.51099831
TATCTCG10150.028.41386241
ATCGGAA10150.028.4066412
GCCGTCT10650.027.41729251
TCGGAAG10650.027.0718963
CCGCTCA11100.026.91499733
ATCTCGT11200.025.75071742
CGTCTGA11500.025.37208616
Produced by FastQC (version 0.11.5)