FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7635162
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78970.10342937058833854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG500.00749251427.78264632
AGGAGCG8050.00494968057.0943270
AGGGGGG30550.06.92733532
CCCCCGG10852.564362E-46.84258670
GAGCGGG6753.400687E-46.68652739
TCGAGTA5850.0037289646.541705664
AGAGCGG6650.00209335216.26482968
TAGGGGG14204.0363375E-85.872966350
AGCGGGG8902.4629995E-45.85174110
TGGGGGG55450.05.8187412
Produced by FastQC (version 0.11.5)