FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8601662
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAA3100.040.3301135
TCCGCGA3100.039.2084634
CGCGAAA3600.033.76519836
CTCCGCG4000.030.38567533
TATGCCG4350.027.96296548
CGTATGC5600.022.33858546
CTCGTAT5000.021.54144344
GCGAAAT6150.021.46000937
TGCCGTC6000.020.85772350
TCTCGTA5350.020.1317243
ATGCCGT7150.018.95957849
GATCGGA6600.018.4185581
GCCGTCT7150.017.9912751
ACTCCGC7900.017.14325332
ACACGTC7800.016.4714813
CGTCTTC8350.016.24193853
CGTCTGA7950.015.7239216
TCGTATG8550.014.63009945
CACTCCG9250.014.26568431
ATCTCGT8200.013.55786942
Produced by FastQC (version 0.11.5)