FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6850942
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4250.032.70755448
CTCGTAT5200.026.72784444
CGTATGC5200.026.06250246
TGCCGTC5700.025.60825550
TCTCGTA5600.025.43843543
GTCACGA5750.022.95465729
ATGCCGT6500.021.38617949
ATCTCGT6450.021.00871342
TCGTATG6700.020.74505845
CGTCTGA6700.020.73642516
GATCGGA6200.020.7326051
CGTCTTC7000.020.35877653
GCCGTCT6700.020.23071751
ACACGTC7300.019.03192513
CCGTCTT7500.018.07396352
ATCGGAA7550.017.9437792
ACGAGAT7800.017.81335632
CGCGGAC1401.979522E-417.3670129
TCGGAAG8000.016.934443
ACGTCTG8500.016.7538115
Produced by FastQC (version 0.11.5)