FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667181
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122170.18324086296742206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG51900.046.7274551
TATGCCG2504.5474735E-1119.46325748
CGTATGC3050.019.36918646
GATCGGA3057.8034645E-1015.9482221
CTCGTAT3253.081732E-813.89909244
GCCGTCT3851.4151738E-913.54417651
ATCGGAA4852.764864E-1012.1764182
TCTCGTA3752.0006883E-712.04506743
TCGTATG4054.186768E-812.01192145
CGTCTGA5552.6047928E-910.63865816
TCGGAAG5704.0345185E-910.3597033
ACACGTC5704.0399755E-910.35884913
TGCCGTC4703.262503E-710.354506550
GTCACGA5401.927765E-810.29098929
ATGCCGT4703.6456586E-69.61410549
ACGTCTG8202.3119355E-98.47124215
AGTCACG6402.4541114E-68.14033428
ATCTCGT6009.035641E-68.10695242
CGTCTTC6251.5431768E-57.78857753
ACGAGAT7251.7483817E-67.665358532
Produced by FastQC (version 0.11.5)