FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667181
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70280.10541186747442435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG31300.09.5391662
GAGGGGG36950.07.60834071
TGGGGGG49800.06.7623672
GTGGGGG53800.05.54798841
CCCGGCC24601.0534131E-65.235450770
Produced by FastQC (version 0.11.5)