FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6484597
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2950.030.59746637
GCGCATC3350.027.9822938
TCGCGCA3150.027.5524536
TCTCGCG3500.026.7787134
CGTATGC3900.024.05296346
CTCGCGC3900.024.03291735
TCTCGTA4000.023.44330843
TATGCCG4200.021.51515248
CTCGTAT4450.020.29467844
TCGTATG4800.020.26496945
TGCCGTC4500.020.08686350
GCCGTCT5000.018.08138851
CGCATCT5250.017.85619239
GATCGGA4900.017.7133851
ATGCCGT5300.017.05185149
ACTCTCG5350.016.21934132
ATCTCGT5950.015.75874842
CGTCTGA6200.014.55375416
ACACGTC6350.014.20996613
CGTCTTC6500.013.913653
Produced by FastQC (version 0.11.5)