FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4853676
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88110.18153251267698955No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA51500.10610514587294248TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7650.051.8058948
CTCGTAT8050.048.33996644
TCTCGTA7950.048.06599843
TTACTCG8450.047.23832335
CGTATGC8450.046.8885846
ATGCCGT8500.046.63495349
TGCCGTC8450.046.50517750
GCCGTCT8650.045.83763551
GATCGGA8400.045.4421731
ACACGTC9000.044.3254413
TACTCGA8850.044.3243536
ATCGGAA9250.043.5114632
TCGTATG9150.043.29610445
CGTCTGA9550.042.49914616
TCGGAAG10100.040.1923073
CGTCTTC9900.039.7135453
ATCTCGT9900.038.24083742
CGGAAGA10650.037.7893034
CACGTCT10650.037.7838414
ACGTCTG11100.036.56458315
Produced by FastQC (version 0.11.5)