FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4853676
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN112270.23130921800301463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA4652.7285023E-710.48982461
CGGGTGA3450.00166853479.08900861
GGGCGGG12700.09.04645958
AATGCCG3500.0019793258.91997330
GGGGGGC16450.08.0355755
GGGGGCG11751.8189894E-127.99501856
AGAGCGG4450.00197620257.7927358
GGGGCGG13303.6379788E-127.32791157
GGGGGGT25900.06.830116714
TAGGGGG10006.6036773E-76.60473550
AGGGGGG20000.06.257441551
GGGGGGA25700.05.6631292
GCGGGGG11803.626172E-45.01777560
Produced by FastQC (version 0.11.5)