FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849708
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218800.170276242853145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG92600.047.586281
GATCGGA7050.024.1535221
TATGCCG6750.023.16874748
CGTATGC7850.020.36329746
ATCGGAA10050.017.9773032
TCGTATG9000.017.76090645
TGCCGTC9150.017.47219350
TCTCGTA8400.017.37425843
CTCGTAT8500.016.35249744
GCCGTCT9100.016.04102551
CGTCTGA11050.016.03365716
ACACGTC12000.015.92280913
ATGCCGT9900.015.79711949
TCGGAAG11250.015.7500243
CGGAAGA13800.012.336084
ACGTCTG14800.012.20580215
ATCTCGT12750.011.7188842
CCGTCTT13600.011.24475152
CACGTCT17000.011.03492814
GCACACG18500.09.57715811
Produced by FastQC (version 0.11.5)