FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5815279
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1400.052.1203544
TCGCGTA1600.045.60216543
ATCGCGT1800.044.3953842
GATCGGA7800.042.311931
AATCGCG2300.042.29511641
CCGGAGA8450.040.69502635
ACACGTC9800.036.5008513
CGTCTGA9600.036.17725416
ATCGGAA9700.035.809222
ACTCCGG10000.034.7325532
CACTCCG10300.033.71976531
CGGAGAA10000.033.69347436
TCGGAAG10700.032.7863433
CTCCGGA10950.031.71868133
ACGTCTG11100.031.60131815
TCCGGAG11000.031.5755934
TATGCCG3600.030.8901248
ATCTCGT4800.028.2296742
CGGAAGA12750.027.7872374
CACGTCT12750.027.7841314
Produced by FastQC (version 0.11.5)