FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6601654
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG1950.042.76014741
TCGCGTA1100.041.06288543
GATCGGA7700.037.440471
ATCGCGT1550.035.86443742
CCGGAGA8850.032.9670535
CACTCCG9200.030.9542831
ACACGTC9200.030.95123113
TATGCCG3850.030.69482648
TCGGAAG9950.030.0155983
ACTCCGG9650.029.87138432
CGCGTAT1551.8189894E-1229.14228644
ATCGGAA10250.028.4599192
CGGAGAA11200.027.29156736
CTCCGGA10950.027.27713633
CGGAAGA11200.026.6654434
TCCGGAG11100.026.59624134
CGTCTGA10900.026.4425616
CGTATGC3550.026.4311846
TCGCGGA809.118214E-526.05913743
TCTCGTA4150.025.11723943
Produced by FastQC (version 0.11.5)