FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6174231
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT4200.018.20257258
CCCGATG4200.017.3734957
TCCGTAC1958.441988E-616.02991125
CCCCGAT4150.015.90825656
CGATGTA5000.015.29140159
CCGTACT2152.1275926E-514.53863926
CGGGATA3851.4206307E-913.54064245
ATCCGTA2457.263463E-512.75870724
GCCCCGA5850.012.47286955
CATCCGG3953.923742E-711.43552941
ATCCGGG4107.573015E-610.17075442
TGCCCCG8001.8189894E-129.98935154
GTGCAGC20300.09.79520670
GTGTTAC17500.09.12882316
TCATCCG4653.3677796E-58.9663840
CATCCGT3500.00195733578.93102223
CCGGGAT5101.029811E-58.858870544
GTCAGTA18250.08.75579735
GGTCAGT17500.08.73078411
GGTAAAC18350.08.71232446
Produced by FastQC (version 0.11.5)