FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9357118
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.015.35685448
TCTCGTA5150.014.16882843
CTCGTAT4950.014.0397944
TGCCGTC5400.012.87345750
GCCGTCT6107.2759576E-1211.39678451
GATCGGA7051.8189894E-1210.8414671
TCGTATG6603.6379788E-1110.53040545
GTCACGA6704.9112714E-1110.36874229
ATCTCGT7453.6379788E-1210.26074742
CGTATGC6807.0940587E-109.7101246
CGTCTTC8657.2759576E-129.24353353
ACACGTC8851.2732926E-119.0272513
AGTCACG9405.456968E-128.86858428
ATGCCGT7504.150934E-98.8050249
CATCTCG9202.910383E-118.68628341
CGTCTGA8051.6316335E-98.62988516
AGCGAGC5950.0033551788.41547770
GCAGCGG10152.1276683E-68.22201870
TCGGAAG8951.373337E-98.150923
ACGTCTG10607.2759576E-117.864593515
Produced by FastQC (version 0.11.5)