FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6118589
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8000.053.42764348
GCGCATC8650.049.38900838
AATGCGC8900.049.16882735
TGCGCAT9050.047.97207337
CTCGTAT9300.046.32343744
TGCCGTC9400.045.47442250
GCCGTCT9100.045.44895651
ATGCCGT9500.045.35822349
TCTCGTA9350.045.33107843
ATGCGCA9600.045.22293536
CGTATGC9500.044.62006846
CGCATCT9600.044.50337239
TAATGCG9900.044.20155734
GATCGGA9950.042.938981
TCGTATG10150.042.44482845
CGTCTGA11150.039.2433416
ATCTCGT10900.038.88237442
ACACGTC11600.038.3187713
TCGGAAG12150.037.7329863
CGTCTTC11300.037.52564253
Produced by FastQC (version 0.11.5)