FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6118589
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84670.13838157784417288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT2150.00499705111.32783959
TAGGGGG10051.8189894E-128.64544150
GGGGGCG15201.8189894E-126.86172756
AGGGGGG22850.06.540696651
GGGGGGT32200.06.46705814
GGGGCGG17850.06.4299357
GGGCGGG16802.910383E-116.21195258
GGGGGGC21653.6379788E-125.620014755
ATAGGGG11703.3004943E-45.04978349
GGGGGGA28300.05.0237512
Produced by FastQC (version 0.11.5)