FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7010904
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84940.12115413361814681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG44000.012.6513562
GAGGGGG45800.011.4714391
TCGACTG2650.002069244210.4394933
TGGGGGG71900.09.5213852
GTGGGGG71350.08.429371
GTGTTCG4100.0078011647.661610657
AGATCGC36650.05.92988370
CAAAGCG18655.635031E-65.82654770
GTGAACC47600.05.406821370
GCAGTGC20402.5378467E-55.326720770
Produced by FastQC (version 0.11.5)