FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6009107
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2900.023.96633548
CGTATGC3350.020.7435246
TCTCGTA3200.020.62612543
GATCGGA3850.017.1392251
TGCCGTC4450.016.40353650
GCCGTCT4550.016.04502351
CTCGTAT4000.015.63373544
ATGCCGT4900.015.60374349
TCGTATG4750.015.36009845
ATCGGAA5600.013.021282
CCGTCTT6250.012.79451352
TCGGAAG6000.012.7317063
CGTCTTC6300.012.69423853
ACACGTC6200.012.31946813
CGTCTGA6000.012.15147516
ATCTCGT5309.640644E-1111.7976742
CGGAAGA7150.011.6551214
TATCGTC2150.00504792111.31245446
ACGTCTG7453.6379788E-119.78642415
TCGCAGG2950.0048653969.41518618
Produced by FastQC (version 0.11.5)