FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6053572
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT600.001772408737.29325570
TATGCCG3500.034.74777248
CGTATGC4700.026.61311546
CGCTCAT4700.025.8657434
ACCGCTC4850.025.06576732
CTCGTAT4300.025.04740744
TATCTCG4300.025.0451341
TGCCGTC4900.024.11229350
ATGCCGT5250.023.16575649
GATCGGA5100.023.1619281
TCTCGTA4900.022.68829743
GTCACCG5300.022.28181529
CCGCTCA5800.022.1578933
GCCGTCT5250.021.84470751
TCGTATG5650.021.52303345
ACACGTC6050.020.6678213
CGTCTGA6300.019.84766616
ATCGGAA6450.019.3889772
ATCTCGT6150.019.20602442
CACCGCT6150.019.20253231
Produced by FastQC (version 0.11.5)