FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6781903
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC4700.040.6441438
TATGCCG5150.035.75892348
GATCGGA5150.035.0705071
AATGCGC5600.033.4883735
CTCGTAT5350.033.11707744
CGTATGC5900.032.3875746
ATGCGCA5900.032.37538536
TGCGCAT5800.031.73716737
TAATGCG5600.031.62743834
TCTCGTA6200.029.1360943
TCGTATG6450.029.08611545
CGCATCT6350.028.98956339
TGCCGTC6450.028.01629450
ATGCCGT6700.027.48795949
GCCGTCT6850.027.3965551
AGGGGGG23800.026.706691
ATCGGAA7550.026.2191942
TCGGAAG7850.024.7738693
ATCTCGT7850.023.01143542
CATCTCG8500.022.88496641
Produced by FastQC (version 0.11.5)