FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6381699
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85110.13336573849691125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5950.045.5625448
CTCGTAT6500.040.62506544
CGTATGC6900.040.29052746
TCTCGTA6950.037.99257743
CGGCTAT7150.037.8805634
TCACCGG7550.036.3258730
TGCCGTC7750.035.88453350
ACCGGCT7650.035.401132
ATGCCGT7950.034.97765449
TCGTATG7950.034.96584345
GATCGGA7700.034.7243961
GCCGTCT8250.033.29043651
CACCGGC8750.031.34649731
GTCACCG9000.030.85692829
ATCTCGT8550.030.4741142
AGGGGGG25050.030.2192381
ATCGGAA9100.030.141572
ACACGTC9300.029.4878113
CGTCTGA9550.028.71587816
CGTCTTC9950.028.6580353
Produced by FastQC (version 0.11.5)