FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447807
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77050.17323143742523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG36300.040.4412651
ATCGGGG3350.038.29081742
GATCGGA3500.037.590761
ATCGGAA3850.035.0660062
AATCGGG4750.029.187841
CGTCTGA4300.028.97468616
GAATCGG4350.028.67713240
ACACGTC5200.025.95648813
CCGGAGA5250.024.40293735
TCGGAAG5550.024.323153
CGGAGAA5500.023.29686236
CGGAAGA5950.022.687984
CACTCCG6400.022.17441231
ACGTCTG7100.018.03549615
ACTCCGG7350.017.89633232
TCCGGAG7800.017.75521934
CACGTCT7850.016.31235714
CTCCGGA8450.015.97789933
TCGGGGG5500.015.76330443
CACACGT9250.015.34005312
Produced by FastQC (version 0.11.5)