FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6460742
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3001.071021E-815.06099948
TGGGTCG3600.007460529510.73362470
CTCGTAT3451.5288107E-410.07323344
CGTATGC3955.940484E-48.79861646
GATCGGA4400.00173213477.8979021
TCGTATG4855.171368E-47.88228945
TGCCGTC5108.841536E-47.496598750
TATCTCG4850.0044945947.165107341
GCCGTCT5000.00603124176.95144551
CCGTCTT6550.00174178786.367982452
CGGAAGA8550.0057093195.2832524
Produced by FastQC (version 0.11.5)