FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5661334
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG2400.00620588213.9072470
CCGCATA2350.00977035410.35027215
TATCCCG3652.6834055E-49.52587764
CGGAACC3100.00725551468.96856143
CAGCGGA3900.0051793728.0196840
CCCGGAT5150.0075706386.79005767
CAACCCG10705.1267433E-45.1960487
ATACCAT38901.2732926E-105.005176570
Produced by FastQC (version 0.11.5)