FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110182
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178620.19606633544752453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG72750.044.0685121
CGCGTAT809.24988E-526.00440244
TGCGCAT5750.024.0935137
GCGCATC5550.023.71639438
TCGCGTA902.059759E-423.11082643
ATGCGCA6200.022.9011436
TATGCCG4100.021.16659748
AATGCGC6750.021.03315735
CGCATCG2553.6379788E-1220.37845239
TAATGCG6650.020.30600234
GATCGGA7000.018.7947251
CTCGTAT4250.018.7639644
ATCGGAA7350.018.3677562
CGTATGC5250.016.51936546
TCTCGTA4450.016.35934843
ACACGTC8500.016.28870413
CGTCTGA10150.015.00437116
TCGGAAG10700.013.9102493
ATCGCGT2253.3314882E-513.86336942
CATCGCG3052.2129461E-713.63422641
Produced by FastQC (version 0.11.5)