FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5047168
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4850.041.56620848
CGTATGC5000.041.00583346
TCGTATC5000.040.9806238
GATCGGA4900.039.6824071
CTCGTAT5350.038.31826444
TCTCGTA5350.038.3144643
TATCTCG5750.036.8535341
TGCCGTC5500.036.6600250
GCCGTCT5650.036.3063651
ATGCCGT5750.035.6692849
GTCACGA5750.035.01501529
CGTATCT6150.032.7570939
TCGTATG6500.031.54106945
ATTCGTA6650.031.33163536
CGAATTC6800.030.63502733
AATTCGT6650.030.28664635
ATCGGAA6950.029.972062
ATCTCGT6850.029.92354442
ACACGTC6850.029.89862813
ACGAATT7100.028.84986532
Produced by FastQC (version 0.11.5)