FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832762
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145220.2125348431571303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGGG8500.017.55716110
GAGCGGG8250.015.564559
AGAGCGG7250.014.83861358
GAGAGCG6900.014.5851947
GGGGGCG17300.011.45629656
TAGGGGG14450.011.06599450
CGGGGGG18350.010.59124812
GGGGGGA36700.010.0159172
CGGGTAA2850.0035868149.76944261
GCGGGTA2850.00359468359.76685260
GCGGGGG18500.09.75491711
GGGGGGC24900.09.63466255
GGGGGGT39500.09.313786514
GGGGCGG21500.09.21988257
AGGGGGG29100.09.07864751
GGGGAGA39850.08.4428044
GGGGGAG38800.08.4018663
GGGCGGG20400.08.35452958
CGGGTGA5058.681478E-58.27017161
GGCGGGT10454.3291948E-107.656747359
Produced by FastQC (version 0.11.5)