FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5867592
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.032.61460548
CGTATGC4600.030.97195646
TCTCGTA4850.027.93392843
GCCGTCT5600.026.70189551
CTCGTAT5250.026.47002444
TGCCGTC5550.026.31296250
GATCGGA5200.026.0344241
CGTCTGA5200.026.0284316
TATCTCG5350.025.3193941
ATGCCGT6050.022.4105349
ATCTCGT5900.022.37232242
ACACGTC6100.022.1881713
ATCGGAA6500.021.359032
CCGTCTT6800.020.45859552
CGTCTTC6650.020.40072853
TCGGAAG6850.020.2675193
TCGTATG6900.020.14264145
ACGTCTG8550.016.2360615
CGGAAGA8350.016.2107054
CACGTCT9200.015.08894514
Produced by FastQC (version 0.11.5)