FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7033037
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5050.029.57928544
GATCGGA5400.028.3042051
TATGCCG5200.028.06477748
CGTATGC5450.026.77340146
TCTCGTA5700.026.20527643
ATCGGAA6350.025.1594982
TCGTATG6150.024.28937145
TGCCGTC6350.023.53255750
GCCGTCT6350.022.98659751
TCGGAAG7850.021.6792533
ATGCCGT7200.021.23510449
ACACGTC7400.021.11707713
CGTCTGA7950.019.65614916
ATCTCGT7800.018.7037342
CGTCTTC8550.017.88728553
CCGTCTT8350.017.48194552
ACGTCTG9750.016.73964715
CGGAAGA9250.016.5198944
CACGTCT10500.014.88251114
AATCTCG11000.013.26226641
Produced by FastQC (version 0.11.5)