FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7822753
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5600.037.24051348
CTCGTAT6250.032.24802444
CGTATGC6900.031.22805846
TCGTATG7650.028.61931645
TCTCGTA6450.028.5526443
GATCGGA8150.025.573841
ATGCCGT7900.025.52002749
TTACTCG8400.025.22529235
TGCCGTC8200.024.16401350
ATCGGAA9150.023.15722
GCCGTCT8950.022.5282251
TACTCGA8650.022.48978636
CGTCTTC9450.022.07495353
ACACGTC10050.021.77133813
CCGTCTT9800.020.22093852
TCGGAAG10850.020.1690433
ATCTCGT8950.019.41180642
CGTCTGA11300.018.74830416
ACGTCTG12200.017.08055715
GCACACG14200.016.1423311
Produced by FastQC (version 0.11.5)