FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979315
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA709.216128E-734.74751343
CGCGTAT1451.1902523E-519.1717944
CCGGAGA8100.017.15515535
GATCGGA7550.017.0255471
TATGCCG4500.016.99136548
CGTCTGA8900.015.61103216
ACACGTC8950.015.5238213
CGTATGC5600.015.5142746
TCGGAAG10100.014.7897053
ATCGGAA9400.014.7825522
TCTCGTA5300.014.42349543
CACTCCG10000.014.2415631
CTCGTAT5650.014.14555644
CGGAGAA9850.014.10752136
AATCGCG2253.253809E-513.89815341
ACTCCGG10850.013.12622732
ATCGCGT2508.741811E-512.50868642
CTCCGGA12950.012.33889733
CGGAAGA11850.012.0193324
TCGTATG7050.011.33701645
Produced by FastQC (version 0.11.5)